WITHDRAWN: Touch DNA localization and direct PCR: An improved workflow for STR typing from improvise explosive devices
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چکیده
منابع مشابه
Str Typing from Bloodstained Fabric without Dna Extraction
Whole blood from various individuals was spotted on 100% white cotton fabric. Once the bloodstains were dry, approximately 1 mm or less of the bloodstained thread was cut from each sample and placed in an autoclaved reaction tube containing either TE buffer or sterile distilled water. The tubes were then subjected to a simple preincubation period as described by the author previously (1). After...
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Human fecal matter is routinely encountered in forensic casework—identifying the source of this material could link a suspect or victim to a crime scene. Previous methods used to identify the source of the fecal matter have primarily involved microscopic comparisons. While these methods can be useful, two sources of fecal matter are required, and typically the analysis cannot individualize the ...
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Improvements in detection limits/sensitivity and lower sample consumption are potential benefits of reducing PCR reaction volumes used in forensic DNA typing of crime scene samples. This premise was studied first with experimental mixtures and a nine-loci megaplex, which demonstrated stochiometric amplification and accurate detection. Next, adjudicated casework samples were subjected to amplifi...
متن کاملAn Efficient Method For DNA Extraction From Paraffin Wax Embedded Tissues For PCR Amplification Of Human And Viral DNA
Background and Objective: Formalin-fixed paraffin-embedded tissues are a valuable source of DNA for molecular studies. We designed and optimized an efficient procedure for DNA extraction from formalin-fixed paraffin embedded tissues. Materials and Methods: Seventy three blocks of cervical paraffin-embedded tissues were investigated. DNA was extracted using 45 minutes boiling in alkaline sol...
متن کاملComparison of cattle BoLA-DRB3 typing by PCR-RFLP, direct sequencing, and high-resolution DNA melting curve analysis
Major histocompatibility complex (MHC) represents an important genetic marker for manipulation to improve the health and productivity of cattle. It is closely associated with numerous disease susceptibilities and immune responses. Bovine MHC, also called bovine leukocyte antigen (BoLA), is considered as a suitable marker for genetic diversity studies. In cattle, most of the polymorphisms are lo...
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ژورنال
عنوان ژورنال: Forensic Science International: Genetics Supplement Series
سال: 2017
ISSN: 1875-1768
DOI: 10.1016/j.fsigss.2017.09.200